abstract：:The polar microenvironment around the reactive Cys283 of rabbit muscle creatine kinase was explored using kinetic analysis of substrates reaction in the presence of modifiers. In the present study, three specific sulphydryl reagents, 5,5'-dithiobis(2-nitrobenzoic acid), 6,6'-dithiodinicotinic acid and 2,2'-dithiodipyr...

journal_title：International journal of biological macromolecules

authors： He HW,Li J,Zhao TJ,Ma Y,Shi F,Zhou HM

更新日期：2007-10-01 00:00:00

abstract：:Immobilization of tyrosinase and alcohol oxidase is achieved in the copolymer of pyrrole with vinyl alcohol with thiophene side groups (PVATh-co-PPy) which is a newly synthesized conducting polymer. PVATh-co-PPy/alcohol oxidase and PVATh-co-PPy/tyrosinase electrodes are constructed by the entrapment of enzyme in condu...

journal_title：International journal of biological macromolecules

authors： Yildiz HB,Sahmetlioglu E,Boyukbayram AE,Toppare L,Yagci Y

更新日期：2007-08-01 00:00:00

abstract：:The immuno-modulating activities and chemical characteristics of exo-biopolymer (EX-GF) produced by a submerged mycelial culture of Grifola frondosa were studied. The EX-GF was fractionated into EX-GF-Fr.I, II, and III by Sephadex G-100 gel chromatography. Anti-complementary activity of EX-GF-Fr.III was highest (71.1%...

journal_title：International journal of biological macromolecules

authors： Yang BK,Gu YA,Jeong YT,Jeong H,Song CH

更新日期：2007-08-01 00:00:00

abstract：:A Ser/Thr phosphatase gene cloned from Aspergillus oryzae, aoppt, revealed that the tetratricopeptide repeat (TPR) and catalytic domains of the full-length AoPPT are located at the N- and C-terminal regions, respectively, similar to those of human Ser/Thr phosphatase 5 (PP5) and yeast Ppt1. Four different regions of A...

journal_title：International journal of biological macromolecules

authors： Feng B,Zhao CH,Tanaka S,Imanaka H,Imamura K,Nakanishi K

更新日期：2007-08-01 00:00:00

abstract：:Sodium houttuyfonate analogs (SHAs), CH(3)-(CH(2))(n)-CO-CH(2)-CH(OH)SO(3)Na, (n=6-14) were synthesized and their molecular interactions with renin and angiotensin I converting enzyme (ACE) studied using fluorescence quenching techniques. Unlike renin, inhibition of ACE activity was not directly proportional to the al...

journal_title：International journal of biological macromolecules

authors： Yuan L,Wu J,Aluko RE

更新日期：2007-08-01 00:00:00

abstract：:We have updated the Protein Sequence-Structure Analysis Relational Database (PSSARD) first published in the Int. J. Biol. Macromol. 36 (2005) 259-262 corresponding to 1573 representative protein chains selected from the Protein Data Bank (PDB). In this, the updated and revised PSSARD (Version 2.0), we have included al...

journal_title：International journal of biological macromolecules

authors： Sridhar S,Babu AV,Guruprasad K

更新日期：2007-06-01 00:00:00

abstract：:In this study, glucose oxidase and polyphenol oxidase were immobilized in conducting polymer matrices; polypyrrole and poly(N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide-co-pyrrole) via electrochemical method. Fourier transform infrared and scanning electron microscope were employed to characterize the copo...

journal_title：International journal of biological macromolecules

authors： Cil M,Böyükbayram AE,Kiralp S,Toppare L,Yağci Y

更新日期：2007-06-01 00:00:00

abstract：:The ability of Delftia acidovorans to incorporate a broad range of 3-hydroxyvalerate (3HV) monomers into polyhydroxyalkanoate (PHA) copolymers was evaluated in this study. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] containing 0-90 mol% of 3HV was obtained when a mixture of sodium 3-hydroxybutyrate an...

journal_title：International journal of biological macromolecules

authors： Loo CY,Sudesh K

更新日期：2007-04-10 00:00:00

abstract：:Growth and glycogen production were characterized for Synechocystis sp. strain PCC6803 grown under continuous fluorescent light in four variations of BG-11 medium: either with (G+) or without (G-) 5mM glucose, and with a normal (N+, 1.5 g sodium nitrate/L) or a reduced (N-, 0.084 g sodium nitrate/L) nitrogen concentra...

journal_title：International journal of biological macromolecules

authors： Yoo SH,Keppel C,Spalding M,Jane JL

更新日期：2007-04-10 00:00:00

abstract：:In this paper, the binding of flavonoids (quercetin and rutin) to hemoglobin (Hb) have been investigated by fluorescence, absorption spectroscopy and circular dichroism (CD) spectroscopy. The binding parameters and binding mode between flavonoids and Hb are determined and the results of CD and synchronous fluorescence...

journal_title：International journal of biological macromolecules

authors： Xi J,Guo R

更新日期：2007-03-10 00:00:00

abstract：:(1-->3)-beta-D-Glucans represent highly conserved structural components of cell walls in yeast, fungi, or seaweed. However, it is still unknown how they mediate their effects. The aim of this study was to evaluate both intraperitoneal and oral application of seaweed-derived (1-->3)-beta-D-glucan Phycarine. Phycarine s...

journal_title：International journal of biological macromolecules

authors： Vetvicka V,Dvorak B,Vetvickova J,Richter J,Krizan J,Sima P,Yvin JC

更新日期：2007-03-10 00:00:00

abstract：:Cross-linking agents play an important part in the physical properties of collagen based biomaterials. Oxazolidines are novel aldehydic tanning agents that are widely used to stabilise collagens in the leather industry. The exact mechanism through which they cross-link collagens is, however, not well understood. When ...

journal_title：International journal of biological macromolecules

authors： Choudhury SD,DasGupta S,Norris GE

更新日期：2007-03-10 00:00:00

abstract：:The gelling properties (gel time (t(gel)) and gel strength) of a 10% (w/w) gelatin sol were investigated as a function of genipin (GP) concentration (0-15 mM) and temperature (25-55 degrees C) to discern mechanisms and optimal conditions for fixation. Gel time increased with increasing temperature, reached a maximum, ...

journal_title：International journal of biological macromolecules

authors： Nickerson MT,Patel J,Heyd DV,Rousseau D,Paulson AT

更新日期：2006-11-15 00:00:00

abstract：:The effect of the limited proteolysis by trypsin on selected seed storage 11S globulins (broad bean and pea legumins, glycinin and helianthinin) was studied by high-sensitive differential scanning calorimetry, fluorescence spectroscopy and analysis of proteolysis kinetics. Different behaviour of glycinin and helianthi...

journal_title：International journal of biological macromolecules

authors： Braudo EE,Danilenko AN,Guslyannikov PV,Kozhevnikov GO,Artykova GP,Lapteva NA,Vaintraub IA,Sironi E,Duranti M

更新日期：2006-11-15 00:00:00

abstract：:The interaction of the protoberberine alkaloid palmatine with single and double stranded structures of poly(A) was studied by various biophysical techniques. Comparative binding studies were also performed with double stranded DNA, t-RNA, poly(C).poly(G), poly(U) and poly(C). The results of competition dialysis, fluor...

journal_title：International journal of biological macromolecules

authors： Giri P,Hossain M,Kumar GS

更新日期：2006-11-15 00:00:00

abstract：:Proteins secreted to mammalian cell supernatants are usually in a low concentration and purity, due to the limitation of the expression systems or the presence of a large amount of contaminant proteins from the cell medium. So, initial protein recovery from cell supernatants requires of a highly specific chromatograph...

journal_title：International journal of biological macromolecules

authors： Ordoño D,Enjuanes L,Casasnovas JM

更新日期：2006-08-15 00:00:00

abstract：:UN1 is a membrane glycoprotein that is expressed in immature human thymocytes, a subpopulation of peripheral T lymphocytes, the HPB acute lymphoblastic leukemia (ALL) T-cell line and fetal thymus. We previously reported the isolation of a monoclonal antibody (UN1 mAb) recognizing the UN1 protein that was classified as...

journal_title：International journal of biological macromolecules

authors： de Laurentiis A,Caterino M,Orrù S,Ruoppolo M,Tuccillo F,Masullo M,Quinto I,Scala G,Pucci P,Palmieri C,Tassone P,Salvatore F,Venuta S

更新日期：2006-08-15 00:00:00

abstract：:The graft copolymer, chitosan-g-polyethylene glycol (PEG), was prepared through graft polymerization of PEG chains to chitosan due to the esterification reaction between PEG and 6-O-succinate-N-phthaloyl-chitosan (PHCSSA). The graft copolymer with porous structure was observed from scanning electron micrographs. It is...

journal_title：International journal of biological macromolecules

authors： Huang M,Liu L,Zhang G,Yuan G,Fang Y

更新日期：2006-05-30 00:00:00

abstract：:Identifying protein-protein interfaces is crucial for structural biology. Because of the constraints in wet experiments, many computational methods have been proposed. Without knowing any information about the partner chains, a new method of predicting protein-protein interaction interface residues purely based on evo...

journal_title：International journal of biological macromolecules

authors： Li JJ,Huang DS,Wang B,Chen P

更新日期：2006-05-30 00:00:00

abstract：:Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron micro...

journal_title：International journal of biological macromolecules

authors： Park KE,Jung SY,Lee SJ,Min BM,Park WH

更新日期：2006-05-30 00:00:00

abstract：:The interaction of novel oligo(L-lysine)-shelled dendrimers (G3-PLL) with DNA was studied by means of circular dichroism spectroscopy, dynamic light scattering, and melting behavior of double-stranded DNA. G3-PLLs having various oligo(L-lysine) (PLL) segment (n = 5-40) were successfully synthesized by graft-polymeriza...

journal_title：International journal of biological macromolecules

authors： Higashi N,Uchino A,Mizuguchi Y,Niwa M

更新日期：2006-03-30 00:00:00

abstract：:Small Hsps represent a variation on the theme of protection of proteins from irreversible aggregation by reversible interaction with chaperone proteins. While different sHsps are highly heterogeneous in sequence and size, the common trait is the presence of a conserved alpha-crystallin domain. In addition sHsps assemb...

journal_title：International journal of biological macromolecules

authors： Haslbeck M

更新日期：2006-03-30 00:00:00

abstract：:This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

journal_title：International journal of biological macromolecules

authors： Kukhtina V

更新日期：2006-03-22 00:00:00

abstract：:This work studies specific interactions and compatibility between a legumin and a linear carboxylated polysaccharide using gel permeation chromatography, sedimentation analysis, SDS gel electrophoresis, viscometry and phase analysis measurements. It uses the system water/11 S globulin/CMC as a model. Carboxymethylcell...

journal_title：International journal of biological macromolecules

authors： Antonov YA,Dmitrochenko AP,Leontiev AL

更新日期：2006-02-28 00:00:00

abstract：:Protein phosphatase inhibitor-1 (PPI-1) is a major inhibitor of protein phosphatase 1 (PP1), which regulates signal transduction in many eukaryotic cellular processes. Biophysical studies have shown that PPI-1 has a large Stokes radius and is heat stable, suggesting that it lacks extensive secondary structures. The un...

journal_title：International journal of biological macromolecules

authors： Kim TD

更新日期：2006-02-28 00:00:00

abstract：:Through semi-interpenetration of polymer networks with poloxamer, mechanical properties of chitosan (CS) sponge were increased for wound dressing application. Synthesis of poloxamer macromer was confirmed by proton nuclear magnetic resonance (1H NMR) spectra. Possible interactions between CS and poloxamer in semi-inte...

journal_title：International journal of biological macromolecules

authors： Kim IY,Yoo MK,Kim BC,Kim SK,Lee HC,Cho CS

更新日期：2006-02-28 00:00:00

abstract：:A purified polysaccharide ACDP-2 was isolated from water extract of the stems of Cistanche deserticola. Chemical and spectroscopic analyses indicated that ACDP-2 is a highly branched arabinogalactan polymer that composes of linked d-galactopyranose and d-glucopyranose, which contains predominantly a branching point at...

journal_title：International journal of biological macromolecules

authors： Wu XM,Gao XM,Tsim KW,Tu PF

更新日期：2005-12-30 00:00:00

abstract：:Human serum albumin (HSA) contains three alpha-helical domains (I-III). The unfolding process of these domains was monitored using covalently bound fluorescence probes; domain I was monitored by N-(1-pyrene)maleimide (PM) conjugated with cys-34, domain II was monitored by the lone tryptophan residue and domain III was...

journal_title：International journal of biological macromolecules

authors： Santra MK,Banerjee A,Rahaman O,Panda D

更新日期：2005-12-15 00:00:00

abstract：:Microscopic size particles of the cholesteric double-stranded DNA (RNA) liquid-crystalline dispersions, containing the ions of the rare earth elements in their content, have been obtained for the first time. The properties of these particles differ from those of classical DNA cholesterics noticeably. The local concent...

journal_title：International journal of biological macromolecules

authors： Yevdokimov YM,Salyanov VI,Kondrashina OV,Borshevsky VI,Semenov SV,Gasanov AA,Reshetov IV,Kuznetsov VD,Nikiforov VN,Akulinichev SV,Mordovskoi MV,Potashev SI,Skorkin VM

更新日期：2005-12-15 00:00:00

abstract：:In this study, a new matrix for immobilization of acetylcholinesterase was investigated by using alginate and kappa-carrageenan. The effects of pH, temperature, storage and thermal stability on the free and immobilized acetylcholinesterase activity were examined. Maximum reaction rate (V(max)) and Michaelis-Menten con...

journal_title：International journal of biological macromolecules

authors： Sahin F,Demirel G,Tümtürk H

更新日期：2005-11-15 00:00:00

abstract：:Collagen, a unique connective tissue protein finds extensive application as biocompatible biomaterial in wound healing, as drug carriers, cosmetics, etc. A work has been undertaken to study the stabilization of type I collagen using the plant polyphenol catechin. Catechin treated collagen fibres showed a shrinkage tem...

journal_title：International journal of biological macromolecules

authors： Madhan B,Subramanian V,Rao JR,Nair BU,Ramasami T

更新日期：2005-10-30 00:00:00

abstract：:The giant tubeworm Riftia pachyptila lives at deep-sea hydrothermal vents along the East Pacific Rise and the Galapagos Rift. The large size and high growth rate of R. pachyptila is supported by an endosymbiotic relationship with a chemosynthetic bacterium. Elucidation of the regulation of energy metabolism of the gia...

journal_title：International journal of biological macromolecules

authors： Uda K,Tanaka K,Bailly X,Zal F,Suzuki T

更新日期：2005-10-30 00:00:00

abstract：:The single Cys residue in the C-terminal domain of bovine eye lens alpha-crystallin was covalently labelled with 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone. This novel SH-reactive two-band ratiometric fluorescent dye is characterized by excited state intramolecular proton transfer reaction yielding two highly emiss...

journal_title：International journal of biological macromolecules

authors： Avilov SV,Bode C,Tolgyesi FG,Klymchenko AS,Fidy J,Demchenko AP

更新日期：2005-09-28 00:00:00

abstract：:Polysaccharides, extracted from the herbs of Chinese natural Solanum lyratum Thunb (SLT), which is a traditional Chinese medicine with hot water and 0.04 M sodium hydroxide successively, were fractionated and purified by ion-exchange and gel-filtration chromatography. According to methylation, periodate oxidation, NMR...

journal_title：International journal of biological macromolecules

authors： Yalin W,Yuanjiang P,Cuirong S

更新日期：2005-09-15 00:00:00

abstract：:A polysaccharide YCP was prepared from a marine filamentous fungus Keissleriella sp. YS4108, which exhibited as a molecular weight (Mw) of 2.4x10(3) kDa and its three sulfated derivatives (YCP-SL, YCP-SM and YCP-SH) were synthesized, the degree of substitution (DS) of which were determined to be 0.13, 0.99 and 1.3, wi...

journal_title：International journal of biological macromolecules

authors： Han F,Yao W,Yang X,Liu X,Gao X

更新日期：2005-09-15 00:00:00

abstract：:It is already known that transcriptional activation of genes occurs due to the H1 dissociation from linker DNA; hence, histone H1-DNA complex is considered as a model of chromatin. Anticancer property of saffron and its carotenoids has already been reported. The present study aimed at investigating the effect of saffr...

journal_title：International journal of biological macromolecules

authors： Ashrafi M,Bathaie SZ,Taghikhani M,Moosavi-Movahedi AA

更新日期：2005-09-15 00:00:00

abstract：:Three strains of Spirulina platensis isolated from different locations showed capability of synthesizing poly(3-hydroxybutyrate) [P(3HB)] under nitrogen-starved conditions with a maximum accumulation of up to 10 wt.% of the cell dry weight (CDW) under mixotrophic culture conditions. Intracellular degradation (mobiliza...

journal_title：International journal of biological macromolecules

authors： Jau MH,Yew SP,Toh PS,Chong AS,Chu WL,Phang SM,Najimudin N,Sudesh K

更新日期：2005-08-01 00:00:00

abstract：:Potato tuber starch was genetically engineered in the plant by the simultaneous antisense suppression of the starch branching enzyme (SBE) I and II isoforms. Starch prepared from 12 independent lines and three control lines were characterised with respect to structural and physical properties. The lengths of the amylo...

journal_title：International journal of biological macromolecules

authors： Blennow A,Wischmann B,Houborg K,Ahmt T,Jørgensen K,Engelsen SB,Bandsholm O,Poulsen P

更新日期：2005-08-01 00:00:00

abstract：:We describe the formation and properties of nanoconstruction that consists of the double-stranded DNA molecules located at distance of 35-50 A in the spatial structure of particles of their cholesteric liquid-crystalline dispersions and cross-linked by artificial nanobridges. The resulting nanostructures possess the p...

journal_title：International journal of biological macromolecules

authors： Yevdokimov YM,Skuridin SG,Nechipurenko YD,Zakharov MA,Salyanov VI,Kurnosov AA,Kuznetsov VD,Nikiforov VN

更新日期：2005-07-01 00:00:00

abstract：:Three well-formed film combinations of chitosan, in the beta form and cellulose acetate biopolymer, having different proportions, have been synthesized and characterized by X-ray diffraction and infrared spectroscopy. The film having a 1.0/0.50 proportion presented 6.87 mmol of nitrogen atoms per gram of synthesized h...

journal_title：International journal of biological macromolecules

authors： Lima IS,Lazarin AM,Airoldi C

更新日期：2005-07-01 00:00:00